TLR4 and diabetes mellitus: In this study, NECA inhibited the relative mRNA transcription level of TLR4 and MYD88 and the expression of TLR4 and MYD88 proteins in DCs of healthy individuals (Figures 2C, D), patients with DM (Figures 3C, D) and DR (Figures 5C, D), and BMDCs (Figures 4C, D).