In a separate study (Liu et al., 2012), screening toxoplasmosis human sera using 2D and LC/MS–MS revealed that 13 proteins recognized by IgG antibodies and 1 protein (ROP2) targeted IgM antibodies from group 1 (IgM negative/IgG positive) and group 2 (IgM positive/IgG negative), respectively. The gene discussed is CD40LG; the disease is toxoplasmosis.