Inflammatory cytokines and enzymes, such as interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-α), nitric oxide (NO), inducible nitric oxide synthase (iNOS), and matrix metalloproteinases (MMPs), show a high correlation with the pathogenesis of hemorrhoids. Here, NOS2 is linked to hemorrhoid.