Tumor analysis one month post boost vaccination, when tumors began to escape immune control, showed an increased portion of PD-1+Tim-3+NKG2A+ CD8 T cells and a reduction of cytokine secretion upon ex vivo restimulation compared to day 20, which goes along with the previously described multi-step modality of T cell exhaustion, progressively leading to attenuated CD8 T cell effector functions [21]. This evidence concerns the gene HAVCR2 and neoplasm.