Because of their higher sensitivity and higher signal intensity, the HCC827-GAS-Luc2, MG-63-GAS-Luc2, and NCI-H1650-GAS-Luc2 cell lines were selected for further study as a PD-L1, CD155, and B7-H3 checkpoint inhibitor cancer cell reporters, respectively. The gene discussed is PVR; the disease is cancer.