In vitro experiments demonstrate that curcumin inhibits the proliferation of liver cancer cells by reducing DJ-1 expression and inhibiting the PTEN/PI3K/AKT signaling pathway [108].In vivo studies suggest that curcumin improves diethylnitrosamine-induced liver cancer by regulating oxidative stress, inflammation, and gut microbiota [109]. The gene discussed is AKT1; the disease is liver cancer.