In our E2-WD model (Figure 1A), RAW cells were stimulated with RANKL and treated with 10 nM of 17β-estradiol (E2), a concentration within the physiological range in mouse serum [38] for 2 days (Days 1 and 2), which was followed by withdrawal (ED-WD) for the subsequent 2 days (Days 3 and 4). This evidence concerns the gene TNFSF11 and Wilson disease.