OM-MOG35–55-specific CD4+ T cells derived from the patients exhibited significantly increased PD-1 expression compared to control-derived CD4+ T cells when cultured for 33 days with DEXA-DCs and VITD3-DCs and on day 36 under all culture conditions, reaching a maximum of over 80% when cultured with VITD3-DCs (Figure 8A). This evidence concerns the gene PDCD1 and ocular melanoma.