ANO1 is located on chromosome 11q13, which is frequently amplified in malignant tumors [7,8], and is reported to be highly expressed in glioblastoma [9], head and neck squamous cell carcinoma [10], gastrointestinal cancer [11], pancreatic cancer [12], esophageal cancer [13], breast cancer [14], and prostate cancer [15]. This evidence concerns the gene ANO1 and head and neck squamous cell carcinoma.