ASPG and glioblastoma: Regarding glioblastoma cells, a work by Karpel-Massler et al. (2016) on different glioblastoma cells treated with recombinant E. coli L-asparaginase from Sigma Aldrich (Saint Louis, MO, USA) effectively showed that these tumour cell lines are sensitive to ASNase, with IC50 values between 0.1 and 1.55 U/mL [56], while we obtained 1.2 and 0.8 U/mL for the S206C and P40S enzymes, respectively.