CD86 and neoplasm: The expression of MHC class I (p < 0.05, partial η2 = 0.58), MHC class II (p < 0.01, partial η2 = 0.87), CD80 (p < 0.01, partial η2 = 0.83), and CD86 (p < 0.01, partial η2 = 0.69) significantly increased with the pulsation of EL4 tumor lysate with VP-R8 compared to non-pulse treatment in vitro (Figure 3).