Liver biopsy was performed in patients C and E with the clinical suspicion of primary biliary cholangitis because of persistent and marked elevation of alkaline phosphatase (1000–1135 U/L) and gamma-glutamyl transferase (1000–1410 U/L) and a less persistent elevation of aspartate aminotransferase and alanine aminotransferase, respectively. This evidence concerns the gene GPT and primary biliary cholangitis.