Although experimental design is a widely used tool in the optimization of extraction processes whenever several factors may affect the response to be optimized, it has been used less profusely in the extraction of polyphenols for studies in Alzheimer’s disease, and in the studies in which it has been used, the methodology of response surface analysis is used to evaluate parameters related to anti-AChE capacity [67] or in survival studies in in vivo trials [138]. Here, ACHE is linked to Alzheimer disease.