TRH and Anxiety: This peptide has been implicated with anxiolytic effects since its i.v. injection reduces the burying time of animals subjected to the DBT test [17]; also, an increment in the amygdala’s TRH mRNA expression has been observed in animals performing the EPM vs. those yoked and has been positively associated with the time spent in the open arms of the maze, which is interpreted as a sign of reduced anxiety-like levels.