Similarly, Larson et al. conducted a genome-wide CRISPRn screen in U87 cells exposed to EGFR-targeting CAR T cells and identified that loss of genes in the interferon-gamma (IFNγ) receptor signaling pathway, including IFNGR1, JAK1, and JAK2, rendered GBM cells more resistant to CAR T cell cytotoxicity both in vitro and in vivo [66]. The gene discussed is IFNG; the disease is glioblastoma.