In flow cytometry, cocultures of the breast cancer cell lines HS578T_pDFR.2 and MDA-MB-435S_pFDR.1 with M13SV1_miCP wild-type or M13SV1_ASCT2KO_ miCP cells yielded low amounts of green fluorescent cells beneath 0.1%, which were not significantly altered by addition of TNFα (Figure 8A,B). This evidence concerns the gene TNF and breast cancer.