SOD1 and amyotrophic lateral sclerosis: To address whether the Zn coordination to the ALS-linked SOD1 mutants plays a major role in the antibody cocktail recognition, we treated the seven different types of SOD1 mutants with 1 to 4 molar equivalents of Zn ions, and analyzed the antibody cocktail recognition of these mutants with distinct incorporation of Zn ions using indirect ELISA.