As shown in Figure 5, in vehicle (DMSO)-treated WM278 MEN1 KD (5B) and WM793B MEN1 KD (5C) melanoma cells, the TGFβ transcriptional response was enhanced in cells overexpressing WT Menin relative to that in cells harboring the empty vector, whereas no difference was observed in cells overexpressing MEN1 mutants, confirming that the mutants failed to transmit the TGFβ responses. The gene discussed is MEN1; the disease is melanoma.