To this effect, MIN6 β-cells were treated with verapamil or left untreated (control) for 24 h and then challenged by a T2D-cytomix containing IL-1β (50 ng/mL), TNF-α (50 ng/mL), and palmitic acid (500 μM) to mimic the T2D microenvironment. Here, TNF is linked to type 2 diabetes mellitus.