We investigated the overall immune infiltration, as well as the proportion of T cells, CD8+ T cells, Th, and Tregs out of CD45+ cells in tumor biopsies from patients with bladder cancer stratified according to NMIBC/MIBC, as well as in control bladder tissue from patients without bladder cancer (gating strategy in Figure 1A). The gene discussed is CD8A; the disease is urinary bladder carcinoma.