To explore the effect of GEN1 on ovarian cancer pathogenesis, we transiently transfected the GEN1 cDNA plasmid into SKOV3 and OVCA-433 cell lines, which exhibited relatively low baseline expression of GEN1. The increased levels of GEN1 mRNA and protein levels were verified by qPCR and Western blot. The gene discussed is GEN1; the disease is ovarian carcinoma.