Overexpression of RNF26 decreased the protein level but not the mRNA level of TSC1 in ccRCC cells (Fig. 6c, d), and this effect could be attenuated by a proteasome inhibitor (MG132) but not a lysosomal inhibitor (BafA1) (Fig. 6e, f). The gene discussed is TSC1; the disease is nonpapillary renal cell carcinoma.