In addition, the IHC staining assay indicated that the protein level of RNF26 was positively correlated with the levels of VEGFA (Spearman r = 0.4928, P = 0.0017, n = 38) and p-S6K1 T389 (Spearman r = 0.3522, P = 0.0301, n = 38) in the ccRCC tissue microarray (Fig. 4l–n). This evidence concerns the gene RPS6KB1 and nonpapillary renal cell carcinoma.