TP53 and leukemia: For example, through single-cell multi-omics analysis of HSPCs from patients with myeloproliferative neoplasm, a convergent genomic evolutionary pattern of a double-hit TP53 mutation in hematopoietic stem cells was identified, and based on this trajectory, pre-leukemia stem cells ultimately progressing to secondary AML were found to undergo differentiation arrest prior to TP53 mutation occurrence, and the subsequent P53 mutant clones could be selected by inflammation, leading to clonal expansion.135 Conventional colon adenomas can be traced back to originating from colonic stem cell (CSC).