Interestingly, while the cytoplasmic fraction displayed no changes in DOCK8 protein levels (Supplementary Fig. 5a), the nuclear fraction of both MEC1-002 SIGLEC-6 KO and normal donor B cells had higher levels of DOCK8 [2.7-fold, t(4) = 3.3, P = 0.03; and 3-fold, t(8) = −3.77, P = 0.005, respectively] when compared to MEC1-002 WT and B-CLL cells respectively (Supplementary Fig. 5b), indicating that DOCK8 may be localized to cell membrane from the nucleus in Siglec-6 + MEC1-002 WT and B-CLL cells. This evidence concerns the gene ATR and B-cell chronic lymphocytic leukemia.