To determine if DOCK8 is required for Siglec-6 dependent B-CLL migration, we used the CRISPR-Cas9 technique to establish stable knockout of DOCK8 in MEC1-002 cells that was confirmed by western blot analysis (Supplementary Fig. 4a) and confocal microscopy (Supplementary Fig. 4b). This evidence concerns the gene DOCK8 and B-cell chronic lymphocytic leukemia.