Consistent with a role for increased mTORC1 activity in the etiology of AD, we observed increased phosphorylation of T389 S6K1 and T37/S46 4E-BP1 in the brains of Control-fed 3xTg female and male mice relative to Control-fed NTg mice (Fig. 7A–C, Supplementary Fig. 6A–C). This evidence concerns the gene RPS6KB1 and Alzheimer disease.