In addition, E2F1 siRNA and AURKB overexpression plasmids were co-transfected into 786-0 and CAKI-1 cells to assess whether enhanced AURKB expression could rescue the inhibitory effects of E2F1 depletion on the proliferation and migration of ccRCC cells. This evidence concerns the gene E2F1 and nonpapillary renal cell carcinoma.