Finally, to confirm that our biologic was active in plasma from patients with lupus, we showed that the human isoform of the engineered biologic degraded cell-free (cf) genomic DNA (cf-gDNA), cf mitochondrial DNA (cf-mtDNA), MP-gDNA, and MP mitochondrial DNA (MP-mtDNA) in the plasma of patients with lupus and was nonreactive to neutralizing DNASE1L3 and DNASE1 autoantibodies present in the plasma of these patients. This evidence concerns the gene DNASE1 and systemic lupus erythematosus.