Notably, Nb289 and Nb535, which exhibited high homology with A1, not only bound to human and murine CDH17 proteins but also displayed higher binding affinity than A1 for human CDH17 (Figure 1; Figure S8, Supporting Information).[11c] Subsequent applications of Nb289 confirmed its recognition of murine CDH17 highly expressed in the syngeneic colorectal cancer cell line, Colon26, both in vitro and in vivo. This evidence concerns the gene CDH17 and colorectal cancer.