This work has also led to consideration of the concept that PACAP may be mediating its actions in migraine, such as direct actions at the TNC, to stimulate CGRP release where PACAP and CGRP are co‐localized,89 or via its effect on mast cells,67 or at alternative yet‐to‐be identified receptors. The gene discussed is ADCYAP1; the disease is migraine disorder.