Preincubation with antibody lowers cell adhesion by 25-50% when compared to control tests without antibody or with IgG control. RUNX2 regulates OPN production and helps osteosarcoma cells adhere to pulmonary endothelial cells. RUNX2 and OPN are associated with osteosarcoma metastasis [13]. The gene discussed is SPP1; the disease is osteosarcoma.