ASNS and neoplasm: Notably, HCT116 ASNS+/+ tumors from female mice showed a drastic reduced number (61.9% reduction, p < 0.05) of mitotic figures indicated by pHH3 positivity compared to the HCT116 ASNS+/+ tumors from males (Fig. 2E), suggesting that reduced proliferation and mitosis are associated with the observed tumor suppression in the tumor-bearing female mice with HCT116 ASNS−/− xenograft.