A gastrointestinal pathologist conducted histopathological evaluation of representative sections. 5 μm slide sections obtained from the female HCT116 ASNS+/+ and HCT116 ASNS−/− tumor-bearing mice and confirmed that 100% of the tissue slides were derived from tumors indicating a complete tumor penetrance in both HCT116 ASNS+/+ and HCT116 ASNS−/− mouse xenograft; tumors were poorly differentiated carcinoma, mimicking tumor growth characteristics in humans (Fig. 1H). This evidence concerns the gene ASNS and carcinoma.