Considering these limitations, the current study presents a pioneering bispecific short hairpin RNA (shRNA) system meticulously designed to concurrently downregulate two key oncogenic genes, namely, mTOR and STAT3. We encoded the sequence to be transcribed into shRNA within oncolytic adenoviral vectors to ensure efficacious delivery of this bispecific siRNA into cancer cells. The gene discussed is STAT3; the disease is cancer.