EZH2 and glioma: After validating EZH2 expression at the tissue level, we examined EZH2 mRNA and protein expression at the cellular level, revealing higher levels of EZH2 mRNA and protein in glioma cell lines compared to normal astrocytes, with the lowest expression in U87 and the highest expression in U251 cells, which were selected for subsequent experiments (Fig. 1g, h).