APOBEC3A and myeloid leukemia: THP1 (myeloid leukemia) cells with integrated doxycycline (dox)-inducible APOBEC3A transgene (THP1-A3A) and constitutive Cas9 transgene (Appendix Fig. S1a) were transduced with the Brunello guide RNA (sgRNA) lentiviral library which includes multiple sgRNAs for each human gene as well as non-targeting control sgRNAs (Doench et al, 2016).