This time, we validated the results by real-time fluorescence quantitative PCR experiments in diabetes combined with MASLD rat model, and obtained the following results: the mRNA content of SPP1 and collagen IV in diabetes combined with MASLD rat group were higher than that in the control group, which was consistent with the results of our bio-bioinformatics analysis, and once again verified the results of our bioconfidence analyses. Here, SPP1 is linked to diabetes mellitus.