These data provide evidence that IMPDH inhibitor suppresses HNSCC cell progression by prompting GTP-exhaustion nucleolar stress.To further elucidate the biological roles of IMPDH1 in HNSCC, we established HNSCC cells with stable knockdown of IMPDH1 (Fig. S9). The gene discussed is IMPDH1; the disease is head and neck squamous cell carcinoma.