To investigate the role of NCF4 in colorectal cancer, we intraperitoneally injected littermate WT, Ncf4+/–, and Ncf4–/– mice with AOM on Day 0 followed by three rounds of DSS treatment (2%) in the drinking water, with assessment of the tumors in the colons on Day 70 (Fig. 3a). Here, NCF4 is linked to colorectal cancer.