To test if chronic inhibition of PVHSH2B1 neurons causes obesity, Sh2b1‐Cre mice were bilaterally microinjected into the PVH with Cre‐dependent AAV9‐hSyn‐DIO‐Tetanus toxin (Tettox) or AAV9‐hSyn‐DIO‐mCherry (control) vector (Figure S6A, Supporting Information). The gene discussed is RIC8B; the disease is obesity due to melanocortin 4 receptor deficiency.