Previous literature has reported that macrophages are abundant in the brain tumor microenvironment, especially in the SHH subgroup.[25] We found that clusters C6‐C9 had higher accessibility of motifs for SPI1, SPIB, JUN family, and FOS family transcription factors, which are involved in myeloid or lymphoid cell development (Figure S1E, Supporting Information). This evidence concerns the gene SPI1 and brain neoplasm.