Looking at mRNA expression of CAFs markers (Figures 2B–2G), all six samples were positive for IL-6, ACTA2, PDGFRA, VIM, FAP, and TGFB1. HT-29, which are epithelial cancer cells, express none of these markers except TGFB1, which is expressed by most cell types under homeostasis and upregulated in cancer cells.50 Here, PDGFRA is linked to cancer.