INS and infection: As the infection progressed, even in the presence of more GLUT4, a significant drop in RLR surface abundance was evident at later timepoints (lane 5, VSV; lane 4, EMCV), A similar phenomena was also noted in 3T3-L1 myc-GLUT4-GFP reporter cells as early as 60 minutes after insulin stimulation (Supplemental Fig. 9a), suggesting confinement is a transient event and RLRs are eventually relinquished from GLUT4-mediated tethering.