Therefore, this study aims to identify the expressions of IL-6, IL-6R, glycoprotein (gp130), C-reactive protein (CRP), Janus kinase 2 (JAK2), and signal transducer and activator of transcription 3 (STAT3) in CCA tissue microarray (TMA) using multiplex immunofluorescence (mIF). This evidence concerns the gene IL6 and cholangiocarcinoma.