In order to determine the ideal quantity of 293T‐ACE2‐Furin cells for SARS‐CoV‐2 VLP pseudotyped viral infection, a titration of the SARS‐CoV‐2 VLP pseudotyped viruses was performed using varying cell numbers (5.00 × 103–8.00 × 104 per well). This evidence concerns the gene ACE2 and viral infectious disease.