Furthermore, we simultaneously expressed siRNA targeting circMALAT1 and MSI2 plasmid, revealing that increased MSI2 expression effectively counteracts the negative impact on mammosphere formation, colony formation, proliferation, migration, invasion, and cisplatin resistance in ESCC cells resulting from circMALAT1 knockdown (Figures 8A–E and S5–S10). This evidence concerns the gene MSI2 and esophageal squamous cell carcinoma.