The biological activity of new chitosan derivatives towards cytotoxicity HeLa cancer (human cervical carcinoma), HEp-2 (Human Larynx Carcinoma) cell lines and mode of action of (CS-3NPs) on apoptotic pathways in HeLa cancer cell line using annexin V-FITC and propidium iodide were investigated. The gene discussed is ANXA5; the disease is cancer.