According to the KEGG analysis, the DEGs were found to be involved mainly in the PI3K-Akt signaling pathway, calcium signaling pathway, MAPK signaling pathway, etc. Substantial evidence has indicated that the PI3K-Akt signaling pathway is frequently hyperactivated in osteosarcoma and contributes to tumorigenesis, proliferation, invasion, cell cycle progression, inhibition of apoptosis, angiogenesis, metastasis and chemoresistance [37–41]. Here, AKT1 is linked to osteosarcoma.