To identify IFN-responsive cells in the brain, we performed single-cell RNA sequencing (scRNA-seq, 10X Genomics) on cells isolated from the forebrains of GIFN and non-transgenic wild-type (WT) littermate control mice at 4 weeks, prior to the onset of symptomatic neurological disease (Figures 2A, S1C, and S1D). Here, IFNA1 is linked to nervous system disorder.