Protein assays conducted on three selected IVD samples from both the control and IDD groups showed that the protein levels of ALKBH5, Runx2, and the aforementioned MMPs and ADAMTSs were significantly elevated in the IDD group, with YTHDF1 expression notably reduced (Fig. 10T). This evidence concerns the gene YTHDF1 and intervertebral disk degenerative disorder.