As expected, control (NT) PDAC spheroid volume was significantly reduced in the presence of budesonide as well as in the presence of dexamethasone and hydrocortisone; however, this effect was blunted in NR3C1 KD tumor spheroids (Fig. 5F), suggesting that the anti-proliferative effect of budesonide in 3D spheroids relies on the GR. This evidence concerns the gene NR3C1 and neoplasm.