Among them, male patients (P < 0.001), previous smoking history (P < 0.001), previous history of lung disease (P = 0.005), FVC < 80% (P = 0.033), FEV1 < 80% (P = 0.005), FEV1 / FVC < 70% (P = 0.002), ECOG PS score ≥ 2 points (P = 0.049), lobectomy (P = 0.001), tumor diameter ≥ 2.25 cm (P < 0.001), non-adenocarcinoma (P < 0.001), pathological stage I-II (P < 0.001), T2-3 (P = 0.006), N0 and N2 (P < 0.001) and Ki67 expression ≥ 25% (P < 0.001) were higher in the low ALI group. The gene discussed is MKI67; the disease is adenocarcinoma.